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B-type natriuretic peptide

Alternate Name: BNP Atellica

SAL Code:

7533

CPT:

83880

Loinc:

30934-4

Turn Around Time:

24 hours

Setup:

Mon-Sun

Units:

pg/mL

Department:

Special Chemistry

Methodology:

The Atellica IM BNP assay is a fully automated 2-site sandwich immunoassay using direct chemiluminescent technology which uses constant amounts of 2 monoclonal antibodies. The first antibody, in the Lite Reagent, is an acridinium-ester-labeled mouse monoclonal anti-human BNP F(ab’)2 fragment specific to the ring structure of BNP. The second
antibody, in the Solid Phase, is a biotinylated mouse monoclonal anti-human antibody specific to the C-terminal portion of BNP, which is coupled to streptavidin magnetic particles. A direct relationship exists between the amount of BNP present in the patient sample and the amount of relative light units (RLUs) detected by the system.

Specimen Requirements:

Primary Tube:

Lavender

Primary Substance:

Plasma EDTA (FRZN)

Alternate Sample Info:

Uncentrifuged whole blood (EDTA), Separated EDTA Plasma

Temperature

Period

Stable Fridge:

Uncentrifuged whole blood (EDTA) up to 24 hours

Stable Frozen:

Uncentrifuged whole blood (EDTA) up to 9 months

Clinical Info:

The Atellica® IM B-Type natriuretic peptide (BNP) assay is for in vitro diagnostic use in the quantitative determination of B-type natriuretic peptide in human plasma (EDTA) using the Atellica® IM Analyzer. This assay is indicated for the measurement of plasma BNP as an aid in the diagnosis and assessment of the severity of heart failure. In patients with acute coronary syndromes (ACS), this test, in conjunction with other known risk factors, can also be used to predict survival as well as to predict the
likelihood of future heart failure. This assay is not intended for use on any other system.

Test Limitations:

- This test has been evaluated with plasma using EDTA as the anticoagulant. Serum, sodium citrate, lithium heparin, and sodium fluoride sample tubes have also been tested and are not recommended.
- Atellica IM BNP test results should not be used interchangeably with other
manufacturers’ BNP assays, nor should Atellica IM BNP test results be used
interchangeably with NT-proBNP assay results.
- Patient samples may contain heterophilic antibodies that could react in immunoassays to give falsely elevated or depressed results.

Test Information:

Components: